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You cut a gene of interest with HincII and would like to insert it into the multiple cloning site (MCS) of a plasmid. The plasmid's MCS contains the cut sites listed below. Which of these sites could you use to insert the gene of interest? Select all that apply. GTC*GAC 1. CAG*CTG G*TYRAC 2. CARYT*G GT*CGAC 3. CAGC*TG GGT*ACC 4. CCA*TGG GTA*TAC 5. CAT*ATG none of these sites

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The following options are selected as an answer:

  • GTC*AAC CTG*TTG (1)
  • GGT*ACC CCG*TGG (4)
  • GTA*TAC CAT*ATC (5)

In biotechnology, bioengineering, and molecular genetics, multiple cloning sites are a feature that allows for the insertion of foreign DNA without damaging the rest of the plasmid. Only one direction of ligation is conceivable if the DNA is cut with two distinct enzymes at the ends. This is true since each end would require a different ligation sequence. Restrictions enzymes are used to cut the plasmid (blue, backbone) and the DNA sequence of interest (green, insert) to provide compatible overhangs that enable them to bind. Covalent connections between the insert and backbone are created via ligase.

Know more about the multiple cloning sites at: https://brainly.com/question/16015626

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