The direction of the regulation of gene expression is to identify whether the sRNA isolated from bacterial cell is cis-encoded or trans-encoded.
Small Regulatory RNAs:
These are the small RNA molecules (sRNA) used for the gene expression in prokaryotes. They directly bind to the target mRNA and initiate the regulation of the gene expression.There are two types of small regulatory RNA -cis encoded and trans encoded sRNA.
These both sRNA are regulatory proteins, involved in the post-transcriptional regulation in prokaryotes and in eukaryotes.In cis-encoded sRNA, the regulation of gene expression starts in the opposite direction whereas, in trans-encoded the regulation starts in the same direction from the promoter of the bacterial chromosome.
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