The CRISPR-Cas system is a genome engineering tool to edit the genome of any species. The crRNA is a sequence complementary to the target DNA of this genome editing system.
The CRISPR-Cas genome editing system is a recently developed genome engineering tool, which can be used to edit the genome of virtually any species.
This genome editing system consists of a nuclease (Cas) protein that cuts the DNA at the targeted regions, and a single guide RNA (sgRNA), which contains a crRNA (17-20 nt) sequence complementary to the target DNA.
In consequence, the sgRNA guides to the Cas protein in order to cleave the genome at targeted nucleotide sequences.
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