Karlie's prediction regarding the main subject is:
A). how mad cow disease starts in the brain and can affect humans.
The prediction about the key subject is the way cow disease begins by impacting the brain and continues to start impacting humans and their health as well.
This is shown through the details regarding the inadequacy in fixing the walls, as well as, floors of the house resulting in causing harm to human health due to this disease that can negatively damage the brain.
Thus, option A is the correct answer.
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