you are comparing two peptides translated in two different cells. The peptide has an identical primary structure, but there is much less of the peptide produced in one of the cells. Which of the following is a potential explanation?
a. an alternative polyA addition site was selected in one of the cells
b. the length of the polyA tail is different in the two cells
Different lengths of Poly A tails are present for different mRNAs so when we compare two peptides translated in two different cells we have different lengths of PolyA tail
PolyA tail is basically a long chain of nucleotides(adenine nucleotides) which are added to messenger RNA(mRNA) during processing of RNA
It increases the stability of the molecule
It protects the mRNA molecule from undergoing degradation in the cytoplasm
It is involved in binding proteins which further initiates translation;introns are removed from pre mRNA before the final mRNA is exported into cytoplasm
