Acetyl-CoA labeled with 14C in both of its acetate carbon atoms is incubated with unlabeled oxaloacetate and a crude tissue preparation capable of carrying out the reactions of the citric acid cycle. After one turn of the TCA cycle, oxaloacetate would have 14C in:
a. all four carbon atoms.
b. no pattern that is predictable from the information provided.
c. none of its carbon atoms.
d. the keto carbon and one of the carboxyl carbons.
e. the two carboxyl carbons.

Acetyl-CoA labeled with 14C in both of its acetate carbon atoms is incubated with unlabeled oxaloacetate and a crude tissue preparation capable of carrying out the reactions of the citric acid cycle. After one turn of the TCA cycle, oxaloacetate would have 14C in all four carbon atoms.

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shrutiagrawal1798 shrutiagrawal1798 · Answer 2 · 2021-12-15T08:04:13+01:00

The acetyl Co A has been labeled with 14C, thus after one cycle of the TCA cycle, the oxaloacetate has been consisting of 2 carbonyl carbons to be radiolabelled. Thus, option D is correct.

TCA cycle has been the citric acid cycle that has been used for the formation of the energy for the cell. The reaction has been based on the reduction of the acetyl Co A that results in the formation of oxaloacetate along with the energy in the form of ATP and GTP.

The acetyl Co A has been the reactant or the start point of Kreb's cycle. The cycle after one turn has been able to produce 1 oxaloacetate molecule. The Acetyl Co A has been able to give its 2 carbons in order to form 1 oxaloacetate molecule.

The acetyl Co A has been labeled with 14C, thus after one cycle of the TCA cycle, the oxaloacetate has been consisting of 2 carbonyl carbons to be radiolabelled. Thus, option D is correct.

For more information about the TCA cycle, refer to the link:

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