Existing isolated brook trout populations in Newfoundland, Canada, were once part of a larger population that was fragmented at the end of the most recent glaciation period about 10,000 to 12,000 years ago. Researchers investigated 14 naturally separated stream populations of brook trout. They found that the populations are all genetically distinct and show differences in morphology. (a) Describe the prezygotic barrier that results in these genetically distinct populations.