The size of pcDNA3 is 5446 bp, and its concentration is 112 ng/μl.
a. You add 5 μl of pcDNA3 into a single-cut digestion reaction, in which the total volume is set to 20 μl. What is the new concentration of pcDNA3?
b. Calculate the molecular mass of pcDNA3.
c. Calculate the volume you need to obtain 0.1 pmol pcDNA3, based on the values you calculated in Parts a and b."