Identify the effect of each error during a thin layer chromatography experiment.
1. The mobile phase used does not move any of the spots at all.
a. There can be no separation of components
b. Accurate Rf values cannot be determined
c. There will be smears on the TLC plate
2. Pen rather than pencil is used to draw the lines on the plate.
a. There can be no separation of components
b. Accurate Rf values cannot be determined
c. There will be smears on the TLC plate
3. The solvent line is not marked after the TLC plate is removed from the chamber.
a. There can be no separation of components
b. Accurate Rf values cannot be determined
c. There will be smears on the TLC plate
4. The TLC plate remains in the chamber even after the solvent has reached the top of the plate.
a. There can be no separation of components
b. Accurate Rf values cannot be determined
c. There will be smears on the TLC plate